The invention relates to the field of bioinformatics and in particular of biomolecular computing (‘DNA computing’). “Computational genes” comprising nucleic acids are provided which, via autonomous spontaneous self-assembly, can be produced in vivo by means of a biomolecular finite automaton.
Publication number: WO2007096187A2 | Search similar patents
A method for sustainable transgene transcription
The present invention relates to constructs and methods for improving expression of transgenes in plants, animals and humans by introducing introns into the transcript to limit the exon sizes to less than about 60 bp.
Publication number: WO2008138896A1 | Search similar patents
Method of identifying the source of genetic information in dna
The present invention embeds predetermined information in the nucleotide sequence of DNA to identify the source of the genetic information in DNA. In particular, the pattern of a nucleotide sequence that normally does not appear in DNA is correlated with identification information for identifying a source of predetermined genetic information belonging to the DNA, and, in said portion of the DNA other than the gene, the nucleotide sequence that is correlated with the identification information is embedded in the DNA so as not to affect the genetic information in the DNA.
Publication number: US2002037521A1 | Search similar patents
Functional arrays for high throughput characterization of regulatory elements in untranslated regions of genes
This invention provides libraries of expression constructs having different untranslated regions (UTR) from a genome. The expression constructs include transcription regulatory sequences operably linked with a reporter gene and a 5′ UTR, a 3′ UTR or both, wherein the translation of the reporter gene is under the regulatory control of control regions in the UTR sequence. The libraries of this invention are useful for determining the impact of regulatory sequences in the UTRs on translation of open reading frames under a variety of conditions, such as different cellular environments.
Publication number: EP2135080A2 | Search similar patents
Gene expression or activity enhancing elements
The present invention relates to transgenic nucleic acids, expression cassettes, vectors, plant cells, plant organs and plants. The invention also relates to methods for increasing expression or activity of a target gene, particularly in a plant cell or plant organ, and also to uses of recombinant nucleic acids and expression cassettes to increase expression or activity of a target gene or for manufacturing of a vector, plant cell, plant organ or plant. Incidentally, the invention relates to enhancers for achieving increased expression or activity of a target gene, particularly in a plant cell or plant organ, when operably linked to a promoter functional in such plant cell, plant organ or plant. The invention is described herein with reference to the technical field of production of polyunsaturated fatty acids (PUFAs), without being limited to this technical field. For the production of desired molecules in plant cells, e.g. PUFAs, it is frequently required to express a target gene heterologous to the plant cell, or to overexpress a target gene naturally found in said plant cell.
Publication number: WO2015001505A2 | Search similar patents
System and method to obtain oligo-peptides with specific high affinity to query proteins
The present invention relates to a method for constructing oligo-peptides with high binding affinity for a query protein.
Publication number: US2007015189A1 | Search similar patents
A molecular vector identification system
The invention relates to a method for constructing a nucleic acid containing an identification insert, comprising providing a nucleic acid and an identification insert, wherein the identification insert is incorporated into the nucleic acid. The invention also relates to nucleic acids containing identification inserts.
Publication number: WO0214553A2 | Search similar patents
Efficient genetic suppressor elements
Genetic elements that are capable of inhibiting gene function are disclosed.
Publication number: EP1245673A2 | Search similar patents
Compositions and methods for generating conditional knockouts
The present invention provides vectors and methods for the generation of conditional knockout and knockdown cells and animals. Vectors of the invention may be used to knockout or knockdown an endogenous gene and conditionally regulate the expression of an endogenous or ectopic gene. Accordingly, the invention provides vectors and methods useful for the identification of disease-associated genes, generating animal models of disease, and identifying drug candidates.
Publication number: US2004045043A1 | Search similar patents
Computational determination of alternative splicing
The present invention provides methods of determining the presence of an alternative transcript form of a nucleic acid molecule. An mRNA sequence is mapped onto a corresponding genomic DNA sequence to reveal at least one mRNA exon fragment. An expressed sequence tag database is interrogated for expressed sequence tags similar to the genomic DNA sequence to generate a collection of expressed sequence tags. The expressed sequence tags in the collection are clustered. The presence of two or more clusters of expressed sequence tags indicates the presence of an alternative transcript form of the nucleic acid molecule.
Publication number: WO03096256A1 | Search similar patents
Inducible small interfering rna (sirna) expression constructs for targeted gene silencing
The invention relates to vectors for the inducible expression of RNA molecules in eukaryotic, particularly mammalian cells and transgenic animals.
Publication number: EP1583832A2 | Search similar patents
Method for the identification and isolation of strong bacterial promoters
The present invention relates to the identification and the isolation from bacterial genomes of new sequences having strong bacterial promoter activity. The invention also concerns new nucleic acids having strong bacterial promoter activity and their uses for improving RNA and/or protein synthesis using cellular (in vivo) or cell-free (in vitro) expression systems.
Publication number: EP1441036A1 | Search similar patents
Process of producing environmentally safe transgenic organisms
A process of producing a transgenic multi-cellular plant or animal organism expressing a trait of interest and having a controlled distribution of said trait to progeny, wherein said process comprises hybridising a first multi-cellular organism or a cell thereof having a first heterologous DNA sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest and a second multi-cellular organism or a cell thereof having a second heterologous DNA sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, whereby said first and said second heterologous sequences are designed such that said trait of interest arises due to RNA trans-splicing after said hybridation.
Publication number: EP1499734A2 | Search similar patents
Growth differentiation factor-9 regulatory sequences and uses therefor
Isolated GDF-9 regulatory sequences are disclosed, as well as methods of using the sequences to modulate tissue-specific expression of genes. The GDF-9 regulatory sequences include, for example, enhancer and promoter elements that naturally drive transcription of GDF-9 in specific tissues. The GDF-9 regulatory sequences can be derived from the untranscribed upstream (e.g., first 10 kilobases) and downstream regions, and transcribed, untranslated regions of a GDF-9 gene.
Publication number: EP1070134A2 | Search similar patents
Method and systems for processing polymeric sequence data and related information
Methods and systems for organizing, representing and processing polymeric sequence information, including biopolymeric sequence information such as DNA sequence information and related information are disclosed herein. Polymeric sequence and associated information may be represented using a plurality of data units, each of which includes one or more headers and a payload containing a representation of a segment of the polymeric sequence. Each header may include or be linked to a portion of the associated information.
Publication number: EP2612271A2 | Search similar patents